Visual pigment assignments in regenerated retina.

نویسندگان

  • D A Cameron
  • M C Cornwall
  • E F MacNichol
چکیده

Retinas of adult teleost fish can regenerate after injury. Two important issues regarding this phenomenon are the assembly of the regenerated retina and the neuronal images of the visual scene that the regenerated retina produces. Here we report experiments in which the visual pigment content of photoreceptors derived from native and regenerated sunfish retinas was determined by microspectrophotometry. In native retina, there is an apparently perfect correspondence between cone morphology and visual pigment content; all rods contain a middle-wavelength pigment, all single cones contain a different middle-wavelength pigment, and all double cone members contain a long-wavelength pigment. The visual pigments in regenerated rods and double cones were the same as in native retina; however, triple cones, a morphology never observed in native retina, contained the long-wavelength pigment. Moreover, although approximately 60% of regenerated single cones contained the expected middle-wavelength pigment, all other single cones contained the long-wavelength pigment. This mismatch between morphology of regenerated single cones and their visual pigment assignment indicated the following: (1) There is a degree of independence between the mechanisms that establish cone morphology and pigment content during regeneration, which suggests that cone photoreceptor regeneration is not a straightforward recapitulation of the normal cone photoreceptor developmental plan. (2) Although anomalous, the long-wavelength single cones may enable regenerated retina to restore the native spectral sampling of the visual scene.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The gecko visual pigments. The behavior of opsin

The 521-pigment extracted out of the retina of the Tokay gecko has the typical stereospecificity of the vertebrate visual pigments. This is true for the pigment in the chloride-depleted, "blue-shifted" state as well as for the normal pigment with added chloride. While in the chloride-deficient state, pigment regeneration occurred with both 11-cis- and 9-cis-retinals and the regenerated photopig...

متن کامل

Retinal pigmented epithelium does not transdifferentiate in adult goldfish.

The neural retina of adult goldfish can regenerate from an intrinsic source of proliferative neuronal progenitor cells, but it is not known whether the retina can regenerate by transdifferentiation of the retinal pigmented epithelium (RPE), a phenomenon demonstrated in adult newts. In this study, we asked whether following surgical removal of the neural retina in adult goldfish the RPE was capa...

متن کامل

Visual pigment reconstitution in intact goldfish retina using synthetic retinaldehyde isomers

A protocol has been developed for reconstituting visual pigments in intact retinae by delivering synthetic isomers of retinal incorporated in phospholipid vesicles. Calibration curves have been constructed relating the lambda(max) of the native porphyropsins (visual pigments based on 11-cis 3-dehydroretinal) of the rods and four spectral classes of cone in the goldfish, and the equivalent photo...

متن کامل

Properties of the pigment layer factor in the regeneration of rhodopsin.

Rhodopsin (visual purple), the photolabile chromoprotein in the retinal rod cells of most vertebrates, bleaches in the light with the release of unstable orange lipides (1) which appear to be precursors (2, 3) of vitamin A aldehyde (retinene) (4, 5). The aldehyde is ultimately reduced to vitamin A alcohol by an enzymatic process (2), which requires reduced diphosphopyridine nucleotide as coenzy...

متن کامل

Molecular basis of spectral tuning in the newt short wavelength sensitive visual pigment.

Previously we reported the sequence of the member of the short wavelength sensitive 2 (SWS2) family of vertebrate visual pigments from the retina of the Japanese common newt, Cynops pyrrhogaster[Takahashi, Y. et al. (2001) FEBS Lett. 501, 151-155]. Now we have expressed the apopigment and regenerated it with A1 retinal. Its absorption maximum, 474 nm, is greatly red shifted compared to other kn...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of neuroscience : the official journal of the Society for Neuroscience

دوره 17 3  شماره 

صفحات  -

تاریخ انتشار 1997